细胞DNA定量分析手艺相干文献

  • 1、HIGH-RESOLUTION IMAGE CYTOMETRY ON SMEARS OF NORMAL ORAL MUCOSA: A POSSIBLE APPROACH FOR THE EARLY DETECTION OF LARYNGOPHARYNGEAL CANCERS(HEAD & NECK 2004年1月)

    作者:Andreas Neher, Andreas Gschwendtner

    关键字:咽喉癌,咽喉粘膜脱落细胞

    择要:

    The purpose of this study was to investigate the possibility of identifying laryngopharyngeal cancers by nuclear chromatin texture feature analysis of cell nuclei from mucosal scrapings obtained from clinically and cytologically noncancerous areas of the soft palate in patients with cancer. The collective consisted of 68 controls and 77 cases of laryngopharyngeal carcinomas. After Feulgen staining, 3000 cell nuclei were automatically measured using a high-resolution image analyser (CytoSavant Oncometrics, Vancouver, BC, Canada). Texture features were extracted for calculation of a discriminant function, which allows the two groups to be distinguished. Two parameters allowed the two populations to be distinguished. The classifier reached an overall performance of 72.7% sensitivity, 82.4% specificity, a positive predictive value of 80.5%, a negative predictive value of 75.1%, and an area under the receiver operating characteristics (ROC) curve of 0.7754. Our work shows that subtle changes in the chromatin distribution in cell nuclei from ostensibly normal cells in the vicinity of carcinomas are demonstrable in the oral cavity of patients suffering from laryngopharyngeal cancers. It may be possible to develop this method into a valuable clinical tool to reduce the high rate of delayed diagnosis of oral and laryngopharyngeal cancers.


  • 2、用细胞DNA 定量分析要领停止宫颈癌普查的临床研讨(诊断病理学杂志 2004年10月)

    作者:孙小蓉, 汪键

    关键字:宫颈癌筛查

    择要:

    目标探究用细胞DNA定量分析要领停止宫颈癌普查,以进步普查的工作效率和准确性.要领对到场宫颈癌普查的20000名妇女用宫颈刷取材,停止液基薄层制片,离别停止巴氏染色和DNA染色.对巴氏染色片做通例细胞学搜检,对DNA染色片停止主动扫描诊断.效果对通例细胞搜检效果正在LSIL以上病例和全自动DNA倍体剖析体系搜检可见同倍体细胞或同倍体细胞峰的病例,发起进一步做阴道镜搜检和宫颈活检.1 016名妇女做了病理活检.以病理诊断效果为尺度,计算出细胞DNA定量分析要领正在筛查GIN3以上宫颈病变的敏感性为82%,特异性为71%,而通例细胞学方轨则离别为52%和92%.结论细胞DNA定量分析要领正在有条件的低落特异性的状况下,能明显提高宫颈癌普查的阳性检出率.


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