细胞DNA定量分析手艺相干文献

  • 1、宫颈上皮内瘤变接纳CCT、LCT 及DNA 定量细胞学搜检对照剖析(海峡防备医学杂志 2006年5月)

    作者:吴燕萍, 马文霞

    关键字:宫颈上皮内瘤变,液基细胞学

    择要:

    目标:剖析对照运用细胞电脑扫描计算机辅助细胞学检测体系(CCT)、液基细胞学搜检计算机辅助细胞学检测体系(LCT)及DNA定量细胞学检测3种要领对诊断宫颈上皮内瘤变(CIN)的意义。[要领]离别接纳CCT、LCT及DNA定量细胞学检测,对宫颈细胞学非常者或有临床非常者,止阴道镜下活检。对照3种细胞学检测要领的CIN检出率。[效果]CCT和LCT检测取阴道镜下活检病理效果相符率对照,差别有明显性。LCT和DNA定量细胞学检测取阴道镜下活检病理效果相符率对照,差别无明显性。[结论]LCT和DNA定量细胞学检测因为接纳液基薄层制片,较CCT通例宫颈涂片,显着低落假阴性率,并提下了对低度鳞状上皮以上病变的诊断率。DNA定量细胞学可测定DNA含量的改动及检出DNA倍体非常细胞。活期检测可对病变的性子及发展趋势作出客观预计和展望。

  • 2、"DNA Ploidy Compared With Human Papilloma Virus Testing (Hybrid Capture II) and Conventional Cervical Cytology as a Primary Screening Test for Cervical High-Grade Lesions and Cancer in 1555 Patients With Biopsy Confirmation(CANCER 2006年7月)"

    作者:Martial Guillaud, Calum MacAulay

    关键字:宫颈癌,液基细胞学,HPV

    择要:

    Abstract Because 80% of cervical cancers arise in low-resource settings, many inexpensive strategies are being tested. In that spirit, the authors are testing large-scale genomic or DNA ploidy measurements as an inexpensive and semiautomated strategy. Patients entered either a screening or diagnostic study of several optical technologies: quantitative cytology, quantitative histopathology, and fluorescence and reflectance spectroscopy using a point probe, a multispectral digital colposcope, or a combination of the two. We calculated sensitivities, specificities, positive and negative predictive values, and their confidence interval testing conventional cytology, Hybrid Capture (HC) II testing, and DNA ploidy measured on the Feulgen-stained quantitative Pap smear. The current investigation reports on 1555 patients for whom colposcopically directed biopsies were read 3 times by study pathologists. The final histopathologic diagnosis was high grade (cervical intraepithelial neoplasia [CIN] 2, CIN 3, carcinoma in situ [CIS], and cancer) in 16% of patients. Using high-grade squamous intraepithelial lesions (SILs) histopathology as the threshold and gold standard, the sensitivity and specificity, respectively, were: 0.47 and 0.96 for conventional cytology, 0.91 and 0.80 for HC II, and 0.59 and 0.93 for DNA ploidy. The positive and negative predictive values (PPV, NPV) for conventional cytology were 0.70 and 0.90, 0.46 and 0.98 for HC II, and 0.63 and 0.92 for DNA ploidy. DNA ploidy shows comparable sensitivity, specificity, PPV, and NPV values to conventional cytology and HC II. Unlike conventional cytology, DNA ploidy is semiautomated and can be performed in less than 8 hours. Cost effectiveness studies are under way, but in the authors' laboratory DNA ploidy is inexpensive.


    3777.com
  • 3、关于“DNA倍体剖析体系用于宫颈癌及上皮内瘤变的诊断及展望"中若干问题的商讨(中华病理学杂志 2006年7月)

    作者:夏潮涌, 孙小蓉

    关键字:宫颈癌

    择要:

    篇首: 贵刊2005年第34卷第7期435页刊载的"DNA倍体剖析体系用于宫颈癌及上皮内瘤变的诊断及展望"一文,有几个问题尚持有差别见解,情愿提出去取作者商讨.


    澳门金沙
  • 澳门金沙
  • 金沙游戏中央官网首页
  • 3777.com