细胞DNA定量分析手艺相干文献

  • 1、宫颈细胞TBS+DNA 倍体定量检测正在宫颈病变中的运用(中国当代医药 2009年6月)

    作者:樊精深,

    关键字:液基细胞学,宫颈病变

    择要:

    目标:经由过程对宫颈细胞 TBS+DNA倍体定量检测,赶早发明宫颈病变,阻断宫颈癌的发作。要领:对妇科门诊救治的1000例妇女停止宫颈细胞TBS+DNA倍体定量检测,阳性 患者再做宫颈活检。诊断尺度接纳TBS分类体系。效果:1000例妇女宫颈刮片标本,TBS+DNA倍体定量检测阳性53例,阳性率为5.3%,个中不典 型腺细胞(AGCUS)2例,低度鳞状上皮内病变(LSIL)40例,高度鳞状上皮内病变(HSIL)9例,鳞状细胞癌(SCC)2例。53例阳性者停止 宫颈活检,个中轻度不典范腺上皮增生2例,CINⅠ39例,CINⅡ7例,CINⅢ3例,SCC2例。宫颈活检对TBS+DNA倍体检测供应的阳性病变加 以确诊。30~40岁年龄阶段是CIN的多发岁数,发病率为56.6%(30/53)。结论:宫颈细胞TBS+DNA倍体定量测定取病理组织学有肯定的相 闭性。TBS+DNA倍体定量测定作为无创伤检测是门诊筛查子宫颈癌的有用要领。对年青妇女应稀奇存眷宫颈癌的门诊筛查事情。

  • 2、宫颈不典范鳞状上皮细胞DNA倍体剖析的临床意义(中国实行诊断学 2009年6月)

    作者:黄卫彤, 韦莹慧

    关键字:不克不及明白意义的非典型鳞状上皮细胞,宫颈病变

    择要:

     宫颈不典范鳞状上皮细胞(atypical squamous ceell,ASC),是指形状改动较良性回响反映性改动显着,但正在数目和水平上又不足以诊断为鳞状上皮内病变的一组阴道、子宫颈的细胞病理改动。因为ASC病理改动多样,临床转归差别明显,关于ASC病例的诊断及处置惩罚,有的学者主张接纳细胞学随访视察,

  • 3、运用细胞DNA倍体剖析法筛查宫颈癌(磨练医学取临床 2009年12月)

    作者:黄宏思, 黄燕

    关键字:宫颈癌筛查

    择要:

    目标 对照传统细胞学取细胞DNA倍体剖析体系对宫颈病变筛查敏感性,寻觅晚期筛查宫颈癌和癌前病变敏感的要领.要领 将1 206例妇科搜检的工具随机分为传统组争DNA倍体剖析组,离别接纳传统宫颈细胞学取细胞DNA倍体剖析体系停止宫颈晚期病变筛查,对筛查阳性病例停止阴 道镜下活检,并对两组阳性效果停止对照剖析.效果 检测显现DNA倍体剖析组筛查阳性率(4.47%)和宫颈上皮内瘤样变(CIN)检出率(82.22%)均高于对比组(1.32%、25.00%),二者 对照差别有统计学意义(P<0.01).结论 细胞DNA倍体剖析检测要领对宫颈病变筛查阳性率和CIN检出率高于传统宫颈细胞学搜检,是晚期筛查宫颈癌和癌前病变敏感而正确的要领.

  • 4、DNA倍体剖析结合HR- HPV检测正在宫颈癌筛查中的感化(中国妇幼保健 2009年11月)

    作者:焦红丽, 夏潮涌

    关键字:宫颈癌筛查,HPV

    择要:

    正在宫颈癌筛查中,结合运用DNA倍体剖析和高危型人乳头状瘤病毒(HR-HPV)检测,为临床宫颈癌疑似病例、病理诊断有疑义病例的判别诊断供应一种有用的要领。要领:对239例细胞学筛查有非常的病例停止病理组织学搜检,随后再次液基薄层细胞学制片、Feulgen染色,做细胞核DNA倍体剖析。对有HPV熏染的病例,将其搜检效果取DNA倍体剖析效果停止对照。效果:239例患者中114例停止了HPV检测,个中HR-HPV熏染病例55例,泛起异倍体69例,两者阳性检出率均取宫颈病变水平相干;HR-HPV熏染病例中发明大部分有异倍体发生,两者均不受岁数影响。结论:HR-HPV熏染能够致使DNA异倍体的泛起,细胞核DNA倍体剖析和HPV搜检效果又均取病变的水平亲切相干,两者结合可为疾病的筛查、预后的判定供应更多有价值的材料。


  • 5、1210例妇女宫颈液基细胞和DNA倍体检查效果剖析(中国老年学杂志 2009年8月)

    作者:黄卫彤, 曲德英

    关键字:液基细胞学,宫颈病变

    择要:

    宫颈癌是最常见的女性生殖讲恶性肿瘤,近年来凭据临床材料显现,老年妇女宫颈癌多发岁数正在55~70岁,并且病发的临床特性是发明病变较晚,病情较严峻,死亡率下.因而,老年妇女应正视宫颈癌筛查.本文对1 210例妇女停止宫颈液基细胞DNA倍体检测效果剖析.


  • 6、Whole-Slide Imaging Digital Pathology as a Platform for Teleconsultation A Pilot Study Using Paired Subspecialist Correlations(Archives of pathology & laboratory medicine , 2009,133)

    作者:David C. Wilbur, Wolfgang Klietmann

    关键字:运用研讨

    择要:

    CONTEXT: -Whole-slide imaging technology offers promise for rapid, Internet-based telepathology consultations between institutions. Before implementation, technical issues, pathologist adaptability, and morphologic pitfalls must be well characterized. OBJECTIVE: -To determine whether interpretation of whole-slide images differed from glass-slide interpretation in difficult surgical pathology cases. DESIGN: -Diagnostically challenging pathology slides from a variety of anatomic sites from an outside laboratory were scanned into whole digital format. Digital and glass slides were independently diagnosed by 2 subspecialty pathologists. 

    Reference, digital, and glass-slide interpretations were compared. Operator comments on technical issues were gathered. RESULTS: -Fifty-three case pairs were analyzed. There was agreement among digital, glass, and reference diagnoses in 45 cases (85%) and between digital and glass diagnoses in 48 (91%) cases. There were 5 digital cases (9%) discordant with both reference and glass diagnoses. Further review of each of these cases indicated an incorrect digital whole-slide interpretation. Neoplastic cases showed better correlation (93%) than did cases of nonneoplastic disease (88%). Comments on discordant cases related to digital whole  technology focused on issues such as fine resolution and navigating ability at high magnification. CONCLUSIONS: -Overall concordance between digital whole-slide and standard glass-slide interpretations was good at 91%. Adjustments in technology, case selection, and technology familiarization should improve performance, making digital whole-slide review feasible for broader telepathology subspecialty consultation applications.

金沙游戏城
  • 金沙游戏城
  • 金沙9882
  • 3015.com
金沙9882